Ts'ebetso e Phahameng ea Liquid Chromatography/ HPLC
High Performance Liquid Chromatography/HPLC e boetse e tsejoa e le "high-pressure liquid chromatography", "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography", joalo-joalo. Tšebetso e phahameng ea metsi chromatography ke lekala la bohlokoa ea chromatography.E sebelisa mokelikeli e le mohato o tsamaeang 'me e sebelisa tsamaiso ea infusion ea khatello e phahameng ho pompa metsoako e le 'ngoe e nang le polarities e fapaneng kapa metsoako e tsoakiloeng, li-buffers le mekhahlelo e meng e tsamaeang ka likarolo tse fapaneng ho ea mokhahlelong o emeng.Ka mor'a hore likarolo tse kholomong ea chromatographic li arohane, li kena ho detector bakeng sa ho lemoha le ho hlahloba sampole.Mokhoa ona o fetohile theknoloji ea bohlokoa ea karohano le tlhahlobo mafapheng a k'hemistri, meriana, indasteri, agronomy, tlhahlobo ea thepa le tlhahlobo ea molao.
Litšobotsi tsa tšebetso e phahameng ea chromatography ea metsi:
① Khatello e phahameng: Karolo ea selefouno ke mokelikeli.Ha e phalla ka har'a kholomo ea chromatographic, e kopana le khanyetso e kholo.E le hore u fete kapele ka har'a kholomo ea chromatographic, mokelikeli o tsamaisang thepa o tlameha ho hatelloa.
②Ho sebetsa hantle haholo: katleho e phahameng ea karohano.Karolo e emeng le e tsamaeang e ka khethoa ho fihlela phello e ntle ka ho fetisisa ea karohano, e leng makhetlo a mangata ho feta katleho ea karohano ea litora tsa distillation tsa indasteri le chromatography ea khase.
③ Kutlo e phahameng: Sesebelisoa sa UV se ka fihla ho 0.01ng.
④Mefuta e mengata ea lits'ebetso: Ho feta 70% ea metsoako ea lintho tse phelang e ka hlahlojoa ka chromatography ea metsi a phahameng.
⑤ Lebelo la tlhahlobo e potlakileng le sekhahla sa phallo ea mokelikeli o potlakileng: e potlakile haholo ho feta chromatography ea khale ea mokelikeli
Ntle le moo, chromatography ea mokelikeli e sebetsang hantle haholo le likholomo tsa chromatography li ka sebelisoa khafetsa, lisampole ha li senyehe, 'me ho bonolo ho li fumana.Leha ho le joalo, li boetse li na le mathata.Ha li bapisoa le chromatography ea khase, e 'ngoe le e' ngoe e na le melemo ea eona ebile e tlatsana.
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Nako ea poso: Nov-23-2023
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